Actividad antibacteriana del extracto acuoso de Kageneckia lanceolata “lloque” contra Staphylococcus aureus
Fecha
2024
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Universidad Nacional San Luis Gonzaga
Resumen
Esta investigación tuvo como objetivo determinar la actividad antibacteriana del extracto acuoso
de Kageneckia lanceolata (lloque) contra las cepas de Staphylococcus aureus ATCC 2921, ATCC
6538, ATCC 25923 y cepas clínicas (4C,5C,6C). Se obtuvieron los extractos acuosos a partir de
hojas y tallos mediante cocción. En la marcha fitoquímica los metabolitos secundarios
encontrados fueron: compuestos fenólicos, flavonoides, saponinas triterpenoides, lactonas
sesquiterpénicas, quinona y cumarinas. Se determinó la actividad antibacteriana mediante el
método de difusión en agar por excavación en diferentes concentraciones. El extracto acuoso del
tallo presentó mayor actividad contra la cepa clínica 4C mostrando un halo de inhibición de 21,3
mm con un porcentaje de inhibición relativa (PIR) de 68,4 %, mientras que con el extracto de
hojas fue con la cepa clínica 6C con un halo de 18,8 mm y PIR de 57,8 %, al disminuir la
concentración los halos fueron reduciendo su tamaño gradualmente. La concentración mínima
inhibitoria (CMI) del extracto acuoso de tallo para las cepas de S. aureus ATCC 2921, ATCC
6538, ATCC 25923 y cepas clínicas (C4, C5 y C6) fue de 12,5 % y para el extracto acuoso de
hojas la CMI contra las cepas de S. aureus ATCC 2921, ATCC 6538, cepas clínicas 5C y 6C fue
de 50 % y para las cepas ATCC 25923 y cepa clínica 4C fue de 25 %. Se concluye que el extracto
acuoso de K. lanceolata tiene diferentes efectos antibacterianos contra S. aureus, siendo la
concentración de 100 % la que presenta mayor actividad antibacteriana.
This research aimed to determine the antibacterial activity of the aqueous extract of Kageneckia lanceolata (lloque) against the strains Staphylococcus aureus ATCC 2921, ATCC 6538, ATCC 25923 and clinical strains (4C,5C,6C). The aqueous extracts were obtained from leaves and stems by cooking. In the phytochemical march, the secondary metabolites found were: phenolic compounds, flavonoids, triterpenoid saponins, sesquiterpene lactones, quinone and coumarins. The antibacterial activity was determined by the excavation agar diffusion method at different concentrations. The aqueous extract of the stem presented greater activity against the clinical strain 4C, showing an inhibition zone of 21,3 mm with a relative inhibition percentage (PIR) of 68,4 %, while with the leaf extract it was with the clinical strain. 6C of S. aureus a halo of 18,8 mm and PIR of 57.8 %, as the concentration decreased the halos gradually reduced in size. The minimum inhibitory concentration (MIC) of the aqueous stem extract for S. aureus strains ATCC 2921, ATCC 6538, ATCC 25923 and clinical strains (C4, C5 and C6) was 12,5 % and for the aqueous leaf extract the MIC against S. aureus strains ATCC 2921, ATCC 6538, clinical strains 5C and 6C was 50 % and for strains ATCC 25923 and clinical strain 4C it was 25 %. It is concluded that the aqueous extract of K. lanceolata has different antibacterial effects against S. aureus, with the 100 % concentration having the highest antibacterial activity.
This research aimed to determine the antibacterial activity of the aqueous extract of Kageneckia lanceolata (lloque) against the strains Staphylococcus aureus ATCC 2921, ATCC 6538, ATCC 25923 and clinical strains (4C,5C,6C). The aqueous extracts were obtained from leaves and stems by cooking. In the phytochemical march, the secondary metabolites found were: phenolic compounds, flavonoids, triterpenoid saponins, sesquiterpene lactones, quinone and coumarins. The antibacterial activity was determined by the excavation agar diffusion method at different concentrations. The aqueous extract of the stem presented greater activity against the clinical strain 4C, showing an inhibition zone of 21,3 mm with a relative inhibition percentage (PIR) of 68,4 %, while with the leaf extract it was with the clinical strain. 6C of S. aureus a halo of 18,8 mm and PIR of 57.8 %, as the concentration decreased the halos gradually reduced in size. The minimum inhibitory concentration (MIC) of the aqueous stem extract for S. aureus strains ATCC 2921, ATCC 6538, ATCC 25923 and clinical strains (C4, C5 and C6) was 12,5 % and for the aqueous leaf extract the MIC against S. aureus strains ATCC 2921, ATCC 6538, clinical strains 5C and 6C was 50 % and for strains ATCC 25923 and clinical strain 4C it was 25 %. It is concluded that the aqueous extract of K. lanceolata has different antibacterial effects against S. aureus, with the 100 % concentration having the highest antibacterial activity.
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Palabras clave
Kageneckia lanceolata, Staphylococcus aureus, Actividad antibacteriana, Extracto acuoso, Antibacterial activity