Polifenoles totales, flavonoides y actividad antioxidante del extracto hidroalcohólico y etanólico de las semillas Mucuna pruriens “frijol terciopelo”
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2024
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Universidad Nacional San Luis Gonzaga. Vicerrectorado de Investigación. Instituto de Investigación
Resumen
La Mucuna pruriens, es una especie originaria de Asia y/o de Malasia; crece en áreas cultivadas,
en los bordes de los bosques, cerca de los océanos y en suelos arenosos. Actualmente es
encontrada gran parte de África, Antillas, México y Sudamérica, es una especie anual, cuyas
semillas son ricas en compuestos fenólicos y se le atribuye diversas propiedades beneficiosas. el
objetivo del presente estudio fue determinar el contenidos de compuestos fenólicos, flavonoides
y actividad antioxidantes de los extractos alcohólicos e hidroalcohólicos. Se utilizo el método de
Folin Cioacalteu para polifenoles totales, de tricloruro férrico para flavonoides y la actividad fue
determinada por dos métodos DPPH y FRAP. Los resultados mostraron que el contenido de
polifenoles totales de fue de 625mg EAG/g y 126 EAG/g para el extracto etanolico e
hidroalcohólico, en el caso de los flavonoides fue de 73,9 y 65,4 mg EQ/g de extracto etanolico e
hidroalcohólico respectivamente; la actividad antioxidante por el metodo DPHH reporto un valor
de mM/mg de 108,9 y 29,5 mg el extracto etanolico e hidroalcohólico respectivamente y en el
caso de metodo FRAP 49,3mM/mg y 10,6mM/mg para los extractos etanolico e hidroalcohólico,
concluyendo que el extracto etanolico presenta un contenido considerable de polifenoles y
flavonoides y una apreciable actividad antioxidante.
Mucuna pruriens is a species native to Asia and/or Malaysia; it grows in cultivated areas, on the edges of forests, near oceans and in sandy soils. It is currently found in much of Africa, the Antilles, Mexico and South America. It is an annual species whose seeds are rich in phenolic compounds and is credited with various beneficial properties. The aim of this study was to determine the content of phenolic compounds, flavonoids and antioxidant activity of alcoholic and hydroalcoholic extracts. The Folin Cioacalteu method was used for total polyphenols, ferric trichloride for flavonoids and the activity was determined by two methods: DPPH and FRAP. The results showed that the total polyphenol content was 625 mg EAG/g and 126 EAG/g for the ethanolic and hydroalcoholic extracts, in the case of flavonoids it was 73.9 and 65.4 mg EQ/g of ethanolic and hydroalcoholic extracts respectively; the antioxidant activity by the DPHH method reported a value of 108.9 and 29.5 mg mM/mg for the ethanolic and hydroalcoholic extracts respectively and in the case of the FRAP method 49.3 mM/mg and 10.6 mM/mg for the ethanolic and hydroalcoholic extracts, concluding that the ethanolic extract has a considerable content of polyphenols and flavonoids and an appreciable antioxidant activity.
Mucuna pruriens is a species native to Asia and/or Malaysia; it grows in cultivated areas, on the edges of forests, near oceans and in sandy soils. It is currently found in much of Africa, the Antilles, Mexico and South America. It is an annual species whose seeds are rich in phenolic compounds and is credited with various beneficial properties. The aim of this study was to determine the content of phenolic compounds, flavonoids and antioxidant activity of alcoholic and hydroalcoholic extracts. The Folin Cioacalteu method was used for total polyphenols, ferric trichloride for flavonoids and the activity was determined by two methods: DPPH and FRAP. The results showed that the total polyphenol content was 625 mg EAG/g and 126 EAG/g for the ethanolic and hydroalcoholic extracts, in the case of flavonoids it was 73.9 and 65.4 mg EQ/g of ethanolic and hydroalcoholic extracts respectively; the antioxidant activity by the DPHH method reported a value of 108.9 and 29.5 mg mM/mg for the ethanolic and hydroalcoholic extracts respectively and in the case of the FRAP method 49.3 mM/mg and 10.6 mM/mg for the ethanolic and hydroalcoholic extracts, concluding that the ethanolic extract has a considerable content of polyphenols and flavonoids and an appreciable antioxidant activity.
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Palabras clave
Mucuna pruriens, Compuestos biactivos, Folin cioacalteu, DPPH, Bioactive compounds