Caracterización fitoquímica, cuantificación de fenoles totales, flavonoides y actividad antioxidante del extracto etanólico de las hojas de Eriobotrya japonica (níspero japonés)
Fecha
2024
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Universidad Nacional San Luis Gonzaga
Resumen
El estudio examinó las hojas postcosecha de la planta Eriobotrya japonica (níspero japonés). Estas
hojas se secan y se muelen hasta obtener una forma granular en polvo, de color verde, sabor amargo
y olor a suigeneris. Los extractos etanólicos se obtuvieron de hojas secas y trituradas utilizando etanol
como solvente y método de digestión, fraccionados para obtener fracciones de este extracto utilizadas
para la determinación de metabolitos secundarios; determinando la presencia de compuestos
fenólicos, taninos, flavonoides, cumarinas, alcaloides, catequinas y saponinas. El contenido total de
polifenoles del extracto etanólico o fracción A y fracción B se determinó utilizando el reactivo de
Folina Ciocalteu, demostrando que una solución al 1,4% de la fracción A tenía un contenido total de
fenoles equivalente a una solución de ácido gálico en concentración 162. 67 mg de ácido gálico/100
ml y contenido total de flavonoides de 8,36 mg calculado como quercetina. Al igual que la fracción
B, su contenido de polifenoles totales se determinó utilizando una solución al 1,0% de la fracción A
y tuvo un contenido de fenoles totales equivalente a una solución de ácido gálico con una
concentración de 89,19 mg de ácido gálico/100 ml y el contenido de flavonoides totales es de 8,74
mg. expresado como equivalente de quercetina. Extracto: alcohol y fracción B, sí. La capacidad de
inhibir la actividad de los radicales libres DPPH con absorbancias de 1,026, 52,24% y 70,77%,
respectivamente.
The study examined the postharvest leaves of the Eriobotrya japonica (Japanese loquat) plant. These leaves are dried and ground into a granular powder form, green in color, bitter in taste, and suigeneris in smell. Ethanolic extracts were obtained from dried and crushed leaves using ethanol as a solvent and digestion method, fractionated to obtain fractions of this extract used for the determination of secondary metabolites, determining the presence of phenolic compounds, tannins, flavonoids, coumarins, alkaloids, catechins and saponins. The total polyphenol content of the ethanolic extract or fraction A and fraction D was determined using Folina Ciocalteu's reagent, demonstrating that a 1.4% solution of fraction A had a total phenol content equivalent to a solution of gallic acid in concentration 162. 67 mg of gallic acid/100 ml and total flavonoid content of 8.36 mg calculated as quercetin. Like fraction D, its total polyphenol content was determined using a 1.0% solution of fraction D and had a total phenol content equivalent to a solution of gallic acid with a concentration of 89.19 mg of acid. gallic/100 ml and the total flavonoid content is 8.74 mg. expressed as quercetin equivalent. Extract: alcohol and fraction D, yes. The ability to inhibit the activity of DPPH free radicals with absorbances of 1.026, 52.24% and 70.77%, respectively.
The study examined the postharvest leaves of the Eriobotrya japonica (Japanese loquat) plant. These leaves are dried and ground into a granular powder form, green in color, bitter in taste, and suigeneris in smell. Ethanolic extracts were obtained from dried and crushed leaves using ethanol as a solvent and digestion method, fractionated to obtain fractions of this extract used for the determination of secondary metabolites, determining the presence of phenolic compounds, tannins, flavonoids, coumarins, alkaloids, catechins and saponins. The total polyphenol content of the ethanolic extract or fraction A and fraction D was determined using Folina Ciocalteu's reagent, demonstrating that a 1.4% solution of fraction A had a total phenol content equivalent to a solution of gallic acid in concentration 162. 67 mg of gallic acid/100 ml and total flavonoid content of 8.36 mg calculated as quercetin. Like fraction D, its total polyphenol content was determined using a 1.0% solution of fraction D and had a total phenol content equivalent to a solution of gallic acid with a concentration of 89.19 mg of acid. gallic/100 ml and the total flavonoid content is 8.74 mg. expressed as quercetin equivalent. Extract: alcohol and fraction D, yes. The ability to inhibit the activity of DPPH free radicals with absorbances of 1.026, 52.24% and 70.77%, respectively.
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Eriobotrya japonica, Cuantificación de flavonoides totales, Cuantificación de polifenoles totales, Actividad antioxidante, Antioxidant activity